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microscopy image browser (mib matlab 2.84)  (MathWorks Inc)


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    MathWorks Inc microscopy image browser (mib matlab 2.84)
    (A) Flow cytometry analysis of the head kidney leukocytes (HKLs, positive control), and red blood cells (RBCs), after incubation with RPMI 1640 or carboxylate-modified latex beads. Distinct populations of HKLs, RBCs, and latex beads were identified based on their side scatter area (SSC-A) versus forward scatter area (FSC-A) profiles, as shown in the top row. The bottom row illustrates the level of green fluorescence intensity (y-axes), representing the fluorescence of latex beads in the FITC channel, versus FSC-A (x-axes) for the same corresponding experimental conditions presented in the top row. A single gated subpopulation is included in each plot: host cells in the top row and latex bead-associated host cells in the bottom row. We included the proportion/percentage of each subpopulation out of total events next to their corresponding plots which e.g., enumerates the number of phagocytic HKLs or latex bead-associated RBCs. (B) Fluorescence <t>microscopy</t> images of (from left to right): beads only, head kidney leukocytes incubated with beads, and the erythrocytes incubated with beads after 1 hour. These images are derived from the same specimens included in (A) .
    Microscopy Image Browser (Mib Matlab 2.84), supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscopy image browser (mib matlab 2.84)/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    microscopy image browser (mib matlab 2.84) - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Erythrocytes of the common carp are immune sentinels that sense pathogen molecular patterns, engulf particles and secrete pro-inflammatory cytokines against bacterial infection"

    Article Title: Erythrocytes of the common carp are immune sentinels that sense pathogen molecular patterns, engulf particles and secrete pro-inflammatory cytokines against bacterial infection

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1407237

    (A) Flow cytometry analysis of the head kidney leukocytes (HKLs, positive control), and red blood cells (RBCs), after incubation with RPMI 1640 or carboxylate-modified latex beads. Distinct populations of HKLs, RBCs, and latex beads were identified based on their side scatter area (SSC-A) versus forward scatter area (FSC-A) profiles, as shown in the top row. The bottom row illustrates the level of green fluorescence intensity (y-axes), representing the fluorescence of latex beads in the FITC channel, versus FSC-A (x-axes) for the same corresponding experimental conditions presented in the top row. A single gated subpopulation is included in each plot: host cells in the top row and latex bead-associated host cells in the bottom row. We included the proportion/percentage of each subpopulation out of total events next to their corresponding plots which e.g., enumerates the number of phagocytic HKLs or latex bead-associated RBCs. (B) Fluorescence microscopy images of (from left to right): beads only, head kidney leukocytes incubated with beads, and the erythrocytes incubated with beads after 1 hour. These images are derived from the same specimens included in (A) .
    Figure Legend Snippet: (A) Flow cytometry analysis of the head kidney leukocytes (HKLs, positive control), and red blood cells (RBCs), after incubation with RPMI 1640 or carboxylate-modified latex beads. Distinct populations of HKLs, RBCs, and latex beads were identified based on their side scatter area (SSC-A) versus forward scatter area (FSC-A) profiles, as shown in the top row. The bottom row illustrates the level of green fluorescence intensity (y-axes), representing the fluorescence of latex beads in the FITC channel, versus FSC-A (x-axes) for the same corresponding experimental conditions presented in the top row. A single gated subpopulation is included in each plot: host cells in the top row and latex bead-associated host cells in the bottom row. We included the proportion/percentage of each subpopulation out of total events next to their corresponding plots which e.g., enumerates the number of phagocytic HKLs or latex bead-associated RBCs. (B) Fluorescence microscopy images of (from left to right): beads only, head kidney leukocytes incubated with beads, and the erythrocytes incubated with beads after 1 hour. These images are derived from the same specimens included in (A) .

    Techniques Used: Flow Cytometry, Positive Control, Incubation, Modification, Fluorescence, Microscopy, Derivative Assay

    Immunofluorescence of the erythrocytes incubated with carboxylate-modified latex beads (A, B) . Erythrocytes were stained with Hoechst33258 (DNA; blue), CellMask (RBC membranes; red), and incubated with latex beads (Alexa Fluor™ 555; orange). All pictures were also captured and merged using differential interference contrast (DIC). Apart from adherence of beads to the surface of RBCs (A, B) , beads were also internalized within the membrane accompanied by co-staining with a membrane-specific dye (CellMask™) of the RBCs: (C) subcellular presence of beads (indicated by white arrowheads) within an erythrocyte. (D) Erythrocytes forming filamentous membrane extensions in the presence of beads (also indicated by white arrowheads). Scale bars: 10 μm. (E) Transmission electron microscopy (1-4) and the serial block face-scanning electron microscopy (SBF-SEM) (5-6) of the erythrocytes incubated with carboxylate-modified polystyrene latex beads (0.5 μm) for 1h (1), 2h (2-3) or 4h (4). The electron micrographs show the adhesion of the beads to the erythrocytes (1-2) or engulfment of the beads (red arrows) (3-4). Scale bars: 2 μm. (5-6) Segmented portion of 3D SBF-SEM of bead engulfment by erythrocytes. Organelle segmentation is color-coded. Red: erythrocytes, Turquoise: latex beads. Scale bars: 1µm.
    Figure Legend Snippet: Immunofluorescence of the erythrocytes incubated with carboxylate-modified latex beads (A, B) . Erythrocytes were stained with Hoechst33258 (DNA; blue), CellMask (RBC membranes; red), and incubated with latex beads (Alexa Fluor™ 555; orange). All pictures were also captured and merged using differential interference contrast (DIC). Apart from adherence of beads to the surface of RBCs (A, B) , beads were also internalized within the membrane accompanied by co-staining with a membrane-specific dye (CellMask™) of the RBCs: (C) subcellular presence of beads (indicated by white arrowheads) within an erythrocyte. (D) Erythrocytes forming filamentous membrane extensions in the presence of beads (also indicated by white arrowheads). Scale bars: 10 μm. (E) Transmission electron microscopy (1-4) and the serial block face-scanning electron microscopy (SBF-SEM) (5-6) of the erythrocytes incubated with carboxylate-modified polystyrene latex beads (0.5 μm) for 1h (1), 2h (2-3) or 4h (4). The electron micrographs show the adhesion of the beads to the erythrocytes (1-2) or engulfment of the beads (red arrows) (3-4). Scale bars: 2 μm. (5-6) Segmented portion of 3D SBF-SEM of bead engulfment by erythrocytes. Organelle segmentation is color-coded. Red: erythrocytes, Turquoise: latex beads. Scale bars: 1µm.

    Techniques Used: Immunofluorescence, Incubation, Modification, Staining, Membrane, Transmission Assay, Electron Microscopy, Blocking Assay



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    microscopy image browser (mib matlab 2.84)  (MathWorks Inc)
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    MathWorks Inc microscopy image browser (mib matlab 2.84)
    (A) Flow cytometry analysis of the head kidney leukocytes (HKLs, positive control), and red blood cells (RBCs), after incubation with RPMI 1640 or carboxylate-modified latex beads. Distinct populations of HKLs, RBCs, and latex beads were identified based on their side scatter area (SSC-A) versus forward scatter area (FSC-A) profiles, as shown in the top row. The bottom row illustrates the level of green fluorescence intensity (y-axes), representing the fluorescence of latex beads in the FITC channel, versus FSC-A (x-axes) for the same corresponding experimental conditions presented in the top row. A single gated subpopulation is included in each plot: host cells in the top row and latex bead-associated host cells in the bottom row. We included the proportion/percentage of each subpopulation out of total events next to their corresponding plots which e.g., enumerates the number of phagocytic HKLs or latex bead-associated RBCs. (B) Fluorescence <t>microscopy</t> images of (from left to right): beads only, head kidney leukocytes incubated with beads, and the erythrocytes incubated with beads after 1 hour. These images are derived from the same specimens included in (A) .
    Microscopy Image Browser (Mib Matlab 2.84), supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscopy image browser (mib matlab 2.84)/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    microscopy image browser (mib matlab 2.84) - by Bioz Stars, 2026-03
    90/100 stars
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    Image Search Results


    (A) Flow cytometry analysis of the head kidney leukocytes (HKLs, positive control), and red blood cells (RBCs), after incubation with RPMI 1640 or carboxylate-modified latex beads. Distinct populations of HKLs, RBCs, and latex beads were identified based on their side scatter area (SSC-A) versus forward scatter area (FSC-A) profiles, as shown in the top row. The bottom row illustrates the level of green fluorescence intensity (y-axes), representing the fluorescence of latex beads in the FITC channel, versus FSC-A (x-axes) for the same corresponding experimental conditions presented in the top row. A single gated subpopulation is included in each plot: host cells in the top row and latex bead-associated host cells in the bottom row. We included the proportion/percentage of each subpopulation out of total events next to their corresponding plots which e.g., enumerates the number of phagocytic HKLs or latex bead-associated RBCs. (B) Fluorescence microscopy images of (from left to right): beads only, head kidney leukocytes incubated with beads, and the erythrocytes incubated with beads after 1 hour. These images are derived from the same specimens included in (A) .

    Journal: Frontiers in Immunology

    Article Title: Erythrocytes of the common carp are immune sentinels that sense pathogen molecular patterns, engulf particles and secrete pro-inflammatory cytokines against bacterial infection

    doi: 10.3389/fimmu.2024.1407237

    Figure Lengend Snippet: (A) Flow cytometry analysis of the head kidney leukocytes (HKLs, positive control), and red blood cells (RBCs), after incubation with RPMI 1640 or carboxylate-modified latex beads. Distinct populations of HKLs, RBCs, and latex beads were identified based on their side scatter area (SSC-A) versus forward scatter area (FSC-A) profiles, as shown in the top row. The bottom row illustrates the level of green fluorescence intensity (y-axes), representing the fluorescence of latex beads in the FITC channel, versus FSC-A (x-axes) for the same corresponding experimental conditions presented in the top row. A single gated subpopulation is included in each plot: host cells in the top row and latex bead-associated host cells in the bottom row. We included the proportion/percentage of each subpopulation out of total events next to their corresponding plots which e.g., enumerates the number of phagocytic HKLs or latex bead-associated RBCs. (B) Fluorescence microscopy images of (from left to right): beads only, head kidney leukocytes incubated with beads, and the erythrocytes incubated with beads after 1 hour. These images are derived from the same specimens included in (A) .

    Article Snippet: The analysis of the images and the 3D model was done on Microscopy Image Browser (MIB MATLAB 2.84) ( ) software and Amira (Thermo Fisher Scientific, USA) platform for visualization, processing, and analysis of 3D models.

    Techniques: Flow Cytometry, Positive Control, Incubation, Modification, Fluorescence, Microscopy, Derivative Assay

    Immunofluorescence of the erythrocytes incubated with carboxylate-modified latex beads (A, B) . Erythrocytes were stained with Hoechst33258 (DNA; blue), CellMask (RBC membranes; red), and incubated with latex beads (Alexa Fluor™ 555; orange). All pictures were also captured and merged using differential interference contrast (DIC). Apart from adherence of beads to the surface of RBCs (A, B) , beads were also internalized within the membrane accompanied by co-staining with a membrane-specific dye (CellMask™) of the RBCs: (C) subcellular presence of beads (indicated by white arrowheads) within an erythrocyte. (D) Erythrocytes forming filamentous membrane extensions in the presence of beads (also indicated by white arrowheads). Scale bars: 10 μm. (E) Transmission electron microscopy (1-4) and the serial block face-scanning electron microscopy (SBF-SEM) (5-6) of the erythrocytes incubated with carboxylate-modified polystyrene latex beads (0.5 μm) for 1h (1), 2h (2-3) or 4h (4). The electron micrographs show the adhesion of the beads to the erythrocytes (1-2) or engulfment of the beads (red arrows) (3-4). Scale bars: 2 μm. (5-6) Segmented portion of 3D SBF-SEM of bead engulfment by erythrocytes. Organelle segmentation is color-coded. Red: erythrocytes, Turquoise: latex beads. Scale bars: 1µm.

    Journal: Frontiers in Immunology

    Article Title: Erythrocytes of the common carp are immune sentinels that sense pathogen molecular patterns, engulf particles and secrete pro-inflammatory cytokines against bacterial infection

    doi: 10.3389/fimmu.2024.1407237

    Figure Lengend Snippet: Immunofluorescence of the erythrocytes incubated with carboxylate-modified latex beads (A, B) . Erythrocytes were stained with Hoechst33258 (DNA; blue), CellMask (RBC membranes; red), and incubated with latex beads (Alexa Fluor™ 555; orange). All pictures were also captured and merged using differential interference contrast (DIC). Apart from adherence of beads to the surface of RBCs (A, B) , beads were also internalized within the membrane accompanied by co-staining with a membrane-specific dye (CellMask™) of the RBCs: (C) subcellular presence of beads (indicated by white arrowheads) within an erythrocyte. (D) Erythrocytes forming filamentous membrane extensions in the presence of beads (also indicated by white arrowheads). Scale bars: 10 μm. (E) Transmission electron microscopy (1-4) and the serial block face-scanning electron microscopy (SBF-SEM) (5-6) of the erythrocytes incubated with carboxylate-modified polystyrene latex beads (0.5 μm) for 1h (1), 2h (2-3) or 4h (4). The electron micrographs show the adhesion of the beads to the erythrocytes (1-2) or engulfment of the beads (red arrows) (3-4). Scale bars: 2 μm. (5-6) Segmented portion of 3D SBF-SEM of bead engulfment by erythrocytes. Organelle segmentation is color-coded. Red: erythrocytes, Turquoise: latex beads. Scale bars: 1µm.

    Article Snippet: The analysis of the images and the 3D model was done on Microscopy Image Browser (MIB MATLAB 2.84) ( ) software and Amira (Thermo Fisher Scientific, USA) platform for visualization, processing, and analysis of 3D models.

    Techniques: Immunofluorescence, Incubation, Modification, Staining, Membrane, Transmission Assay, Electron Microscopy, Blocking Assay